Flt-3 Ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity and a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.

Expression System

Escherichia coli

Sequence

MTQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLEATAPTA with polyhistidine tag at the C-terminus.

Species

Human

Tag

polyhistidine tag at the C-terminus

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measure by its ability to induce proliferation in BaF3 cells transfected with mouse Flt-3.

The ED₅₀ for this effect is <0.8 ng/mL.

The specific activity of recombinant human Flt-3 Ligand is > 1.5 x 10⁶ IU/mg.

Purity

>95% as determined by SDS-PAGE. Ni-NTA chromatography

Formulation

The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Reconstitution

It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 minutes to ensure sufficient re-dissolved.