Skip to content The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton (kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of p roteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heats, dilute, and add reducing agent before loading.
Features
Broad range: 3.5-245 kDa (Tris-glycine-SDS running buffer)
Ready-to-use: supplied in a loading buffer for direct loading on gels
Easy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye
Sharp bands
Applications
Monitoring of protein migration during SDS-PAGE.
Monitoring of protein transfer onto membranes during Western blots.
Sizing of proteins on SDS-PAGE and Western blots.
100 in stock
100 in stock
The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton (kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of p roteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heats, dilute, and add reducing agent before loading.
Features
Broad range: 3.5-245 kDa (Tris-glycine-SDS running buffer)
Ready-to-use: supplied in a loading buffer for direct loading on gels
Easy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye
Sharp bands
Applications
Monitoring of protein migration during SDS-PAGE.
Monitoring of protein transfer onto membranes during Western blots.
Sizing of proteins on SDS-PAGE and Western blots.
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