Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target DNA or RNA and serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.
During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between the mRNA and other RNA species present in the reaction.
Applications
cDNA synthesis using a Reverse Transcriptase with RNA templates.
DNA synthesis using the Klenow fragment with DNA templates.
DNA probe synthesis for use in Southern, Northern, and in situ hybridization applications.
100 in stock
100 in stock
Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target DNA or RNA and serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.
During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between the mRNA and other RNA species present in the reaction.
Applications
cDNA synthesis using a Reverse Transcriptase with RNA templates.
DNA synthesis using the Klenow fragment with DNA templates.
DNA probe synthesis for use in Southern, Northern, and in situ hybridization applications.
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