OnePCR™ Plus is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ Plus is a pre-mixed solution containing Taq DNA polymerase, PCR reaction buffers, dNTPs, gel loading dyes, and fluorescence dye. OnePCR™ Plus contains the Taq DNA polymerase, which is purified from the E. coli. and exhibits the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR™ Plus also contains the fluorescence dye, which is directly detected on BLooK LED Transilluminator (BK001) or UV epi-illuminator after the DNA electrophoresis. OnePCR™ Plus mixture is supplied at the 2X concentrationto allow 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.