The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

Expression System

Escherichia coli

Sequence

MKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCRwith polyhistidine tag at the C-terminus.

Species

Human

Tag

polyhistidine tag at the C-terminus

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.

The ED₅₀ for this effect is <0.58 ng/mL.

Purity

>95% as determined by SDS-PAGE. Ni-NTA chromatography

Formulation

The protein was lyophilized from a solution containing 20 mM sodium carbonate, pH 9.0.

Reconstitution

It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 minutes to ensure sufficient re-dissolved.